Our lab is interested in the neutrophil (PMN) response to pathogens and in particular the NADPH-dependant oxidase and myeloperoxidase (MPO). My work focuses on the NADPH oxidase response to endotoxin. Endotoxin does not directly activate PMNs but exposure renders them more responsive to future stimulation, a phenomenon known as priming. Stimulation by formyl peptides of endotoxin primed PMN results in 5 to 10 times more superoxide production indicating priming affects the NADPH oxidase. The understanding of the priming process is not completely understood. My research focuses on how priming results in the reorganization of components of the NADPH oxidase at lipid rafts in preparation for the oxidative burst.
Ulland TK, Buchan BW, Ketterer MR, Fernandes-Alnemri T, Meyerholz DK, Apicella MA, Alnemri ES, Jones BD, Nauseef WM, Sutterwala FS. Cutting edge: mutation of Francisella tularensis mviN leads to increased macrophage absent in melanoma 2 inflammasome activation and a loss of virulence. J Immunol. 2010 Sep 1;185(5):2670-4. Epub 2010 Aug 2. PubMed PMID: 20679532; PubMed Central PMCID:PMC2953561.
Moreland JG, Hook JS, Bailey G, Ulland T, Nauseef WM. Francisella tularensis directly interacts with the endothelium and recruits neutrophils with a blunted inflammatory phenotype. Am J Physiol Lung Cell Mol Physiol. 2009 Jun;296(6):L1076-84. Epub 2009 Apr 3. PubMed PMID: 19346432; PubMed Central PMCID: PMC2692798.