Jason Netland |
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| Research Interests | |
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Coronavirus pathogenesis and host response to infection
There are eight open reading frames (ORF’s) in the SARS-coronavirus (SARS-CoV) genome that encode non-structural proteins whose functions are currently unknown. I am currently investigating ORF6, which when expressed in the context of a recombinant mouse hepatitis virus, enhances virulence, converting a non-lethal infection into a uniformly lethal infection. My project aims to uncover the function of ORF6 by developing a reverse genetics system and animal model for SARS-CoV that will allow me to manipulate the genome and assay for changes in pathogenesis. In addition, I will be using biochemical techniques to characterize the ORF6 protein and determine its structure, as this may give insight into its function. |
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| Publications | |
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Zhao, J., Falcón, A., Zhou, H., Netland , J, Enjuanes L, Pérez Breña P, Perlman S. Severe acute respiratory syndrome coronavirus protein 6 is required for optimal replication. J Virol. 2009 Mar;83(5):2368-73. Epub 2008 Dec 17. PubMed PMID:19091867; PubMed Central PMCID: PMC2643704.
Perlman. S., Netland , J. Coronaviruses post-SARS: update on replication and pathogenesis. Nat Rev Microbiol. 2009 Jun;7(6):439-50. Review. PubMed PMID:19430490. Netland , J., Meyerholz, D.K., Moore, S., Cassell, M., Perlman, S. Severe acute respiratory syndrome coronavirus infection causes neuronal death in the absence of encephalitis in mice transgenic for human ACE2. J Virol. 2008 Aug;82(15):7264-75. Epub 2008 May 21. PMID: 18495771 [PubMed - indexed for MEDLINE] Netland , J., Ferraro, D., Pewe, L., Olivares, H., Gallagher, T., Perlman, S. Enhancement of murine coronavirus replication by severe acute respiratory syndrome coronavirus protein 6 requires the N-terminal hydrophobic region but not C-terminal sorting motifs. J Virol. 2007 Oct;81(20):11520-5. Epub 2007 Aug 1. PMID: 17670827 [PubMed - indexed for MEDLINE] Tangudu, C., Olivares, H., Netland , J., Perlman, S., Gallagher, T. Severe acute respiratory syndrome coronavirus protein 6 accelerates murine coronavirus infections. J Virol. 2007 Feb;81(3):1220-9. Epub 2006 Nov 15. PMID: 17108045 [PubMed - indexed for MEDLINE] McCray, P.B. Jr, Pewe, L., Wohlford-Lenane, C., Hickey, M, Manzel, L., Shi, L., Netland , J., Jia, H.P., Halabi, C., Sigmund, C.D., Meyerholz, D.K., Kirby, P., Look, D.C., Perlman, S. Lethal infection of K18-hACE2 mice infected with severe acute respiratory syndrome coronavirus. J Virol. 2007 Jan;81(2):813-21. Epub 2006 Nov 1. PMID: 17079315 [PubMed - indexed for MEDLINE] Pewe, L., Zhou, H., Netland , J., Tangadu, C., Olivares, H., Shi, L., Look, D., Gallagher, T., Perlman, S. A SARS-CoV-specific protein enhances virulence of an attenuated strain of mouse hepatitis virus. Adv Exp Med Biol. 2006;581:493-8. No abstract available. PMID: 17037583 [PubMed - indexed for MEDLINE] Jia, H.P., Look, D.C., Hickey, M., Shi, L., Pewe, L., Netland , J., Farzan, M., Wohlford-Lenane, C., Perlman, S., McCray, P.B. Jr. Infection of human airway epithelia by SARS coronavirus is associated with ACE2 expression and localization. Adv Exp Med Biol. 2006;581:479-84. Review. No abstract available. PMID: 17037581 [PubMed - indexed for MEDLINE] Jia, H.P., Look, D.C., Shi, L., Hickey, M., Pewe, L., Netland , J., Farzan, M., Wohlford-Lenane, C., Perlman, S., McCray, P.B. Jr. ACE2 receptor expression and severe acute respiratory syndrome coronavirus infection depend on differentiation of human airway epithelia. J Virol. 2005 Dec;79(23):14614-21. PMID: 16282461 [PubMed - indexed for MEDLINE] Pewe, L., Zhou, H., Netland , J., Tangudu, C., Olivares, H., Shi, L., Look, D., Gallagher, T., Perlman, S. A severe acute respiratory syndrome-associated coronavirus-specific protein enhances virulence of an attenuated murine coronavirus. J Virol. 2005 Sep;79(17):11335-42. PMID: 16103185 [PubMed - indexed for MEDLINE] Pewe, L.L., Netland , J.M., Heard, S.B., Perlman, S. Very diverse CD8 T cell clonotypic responses after virus infections. J Immunol. 2004 Mar 1;172(5):3151-6. PMID: 14978121 [PubMed - indexed for MEDLINE] |
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