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| Immunobiology of cellular transplants derived from embryonic stem cells | ||||||
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Due to their pluripotency, embryonic stem (ES) cells provide an unparalled source of cells that can be directed towards the generation of any cell type desired. This opens up opportunities for establishing new therapies for manipulating the immune system and for the treatment of degenerative diseases. Our laboratory is interested in understanding the transcriptional regulatory requirements for deriving hematopoietic cells from ES cells. ES cells are coaxed to differentiate into hematopoietic progenitor cells that express low levels of MHC class I and class II antigens, a characteristic that allows for their transplantation across MHC barriers without the need for immunosuppressive therapy. Current topics include:
1. Efficacy of HOXB4 to promote the differentiation ES cells into definitive hematopoietic cells. 2. Role of NK cells in the engraftment of ES-derived hematopoietic progenitor cells and T cell-mediated clonal deletion of allogenic ES-derived cells. 3. Induction of transplantation tolerance by ES-derived hematopoietic cells. 4. De novo generation of T cells from ES-derived hematopoietic progenitor cells. |
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| Selected Publications | ||||||
| Click Here for a Complete List of Articles | ||||||
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Tabayoyong, W.B., Salas, J.G., Bonde, S. and Zavazava N. HOXB4 transduced ES cell-derived Linc-c-kit+ and Lin-Sca-1+ hematopoietic progenitors express H60 and are targeted by NK cells. J. Immunol. (in press).
Bonde, S., Chan, K.M. and Zavazava N. ES-cell derived hematopoietic cells induce transplantation tolerance. Plos One, 3(9): e3212, 2008. Chan, K.M., Bonde, S., Klump, H. and Zavazava N. Hematopoiesis and immunity of HOXB4-transduced embryonic stem cell-derived hematopoietic progenitor cells. Blood 111(6):2953-61, 2008. Bonde, S. and Zavazava N. Immunogenicity and engraftment of mouse embryonic stem cells in allogeneic recipients. Stem Cells. (10):2192-201, 2006. Faendrich, F., Lin, X., Chai, G.X., Huang, D.S., Ganten, D., Bader, M., Schulze, M., Holle, J., Parwaresch, R., Zavazava N. and Binas, B. Preimplantation-stage stem cells induce allogeneic graft tolerance without supplementary host conditioning. Nature Med 8:171-178, 2002. |
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| Department/Program Affiliations |
| Internal Medicine |
| Biosciences |
| Immunology |
| MSTP |